报告题目：国家自然科学基金项目申报与SCI论文撰写的体会
主讲人：花放 教授（徐州医科大学神经系统疾病研究所副所长、江苏特聘教授、博士生导师）
时间：2016年4月18日（星期一）下午13:30
地点：致远楼第一会议室
花放教授简历
江苏特聘教授、博士生导师
美国埃默里（Emory）大学医学院客座副教授
徐州医科大学神经系统疾病研究所副所长
徐医附院脑科医院副院长、教授、主任医师
工作及教育经历：
2012年至今：徐州医科大学神经系统教研室副主任、教授、主任医师
2008/9 - 2012/1，美国埃默里（Emory）大学医学院，助理教授
2002/1 - 2008/8，美国东田纳西州立大学医学院，博士后
2001年7月：中国医科大学博士
1992-2001徐州医学院神经科：讲师、主治医师；副教授；副主任医师
1992年7月：中国医科大学硕士
1983-1989：徐州医学院助教
1983年7月：扬州大学医学院
主持的科研项目及人才计划项目：
1、国家自然科学基金面上项目，黄体酮受体信号通路在新生儿缺氧缺血性脑损伤中的作用、2016/01-2019/12、72万。
2、国家自然科学基金面上项目，81271268、Toll受体信号通路对缺血脑组织中干细胞的调控作用、2013/01-2016/12、75万。
3、江苏省教育厅江苏特聘教授项目，黄体酮受体信号通路与神经系统疾病、2012/09-2015/08、200万。
4、江苏省人力资源厅（六大人才高峰项目），TLRs信号通路与早老性痴呆、2014/01-2015/12、105万。
5、美国心脏与卒中协会科学家发展基金，0830481N、TOLL样受体-4调节的信号转导通路在缺血性卒中的作用、2008/01-2011/12、30万美元。
6、国家自然科学基金面上项目，30970995、TLR-4在缺血性卒中的作用、2010/01-2010/12。
7、美国埃默里大学，2009-002、TOLL样受体2和4在外伤性脑损伤中的作用、2009/07-2010/06
8、美国心脏与卒中协会，0625348B、TOLL样受体-4在脑缺血再灌注损伤中的作用、2006/07-2008/06
9、美国东田纳西州大学科研发展基金，2006-02、TOLL样受体信号转导通路与缺血性卒中、2006/07-2007/06
10、江苏省教委高校重点实验室资助项目，K2002、降钙素基因相关肽对大鼠脑缺血再灌注损伤的保护作用、2001/07-2002/06、3万、已结题、主持。
参加的科研项目及人才计划项目：
1、国家自然科学基金青年基金，81201025、骨髓基质干细胞加重糖尿病脑缺血损伤的新机制-RAGE介导的信号通路、2013/01-2015/12、23万。
2、美国国立卫生研究院，UO1 NS062676-1、黄体酮对缺血性脑损伤的保护作用、2010/01-2013/12、150万美元。
3、美国国立卫生研究院，RO1 HD061971、黄体酮和VDH的联合治疗对脑外伤的保护作用、2009/10-2013/09、120万美元。
4、美国心脏/卒中协会，黄体酮和rt-PA联合治疗急性脑梗死、2010/01-2013/12、30万美元。
5、美国东田纳西州大学科研发展基金，2004-18、脊髓刺激和心脏缺血后脊髓胸段基因表达特征、2004/07-2005/06。
发表论文：
1. Hua F., Liu XM, Li SY.(1994). Observation on efficacy of Vinpocetine in treatment of cerebral infarction of convalescent. Chinese New Drugs Journal, 3: 40-42.
2. Hua F., Liu XM, Li SY. (1995). Study of platelet cytoplasm ionized calcium and calmodulin content in acute cerebral infarction. Journal of Clinical Neurology, 8: 200-202.
3. Shen X, Hua F. (1997). Concentration of GMP-140 in acute cerebral infarction. Acta Academtae Medicinae Xuzhou, 17: 475-476.
4. Hua F. (1997). Cytoplasm ionized calcium concentration of Platelet in migraine. Acta Academtae Medicinae Xuzhou, 17: 482-484.
5. Hua F., Shen X, Liu SL. (1998). He-Ne Laser intravascular irradiation for cerebral infarction: clinical and experimental observation. Chinese Journal of Physical Therapy. 21:14-16.
6. Ding XH, Hua F., Liu WR, Fang XB. (2000). Effect of CGRP on synaptophysin in the hippocampus after cerebral ischemia/reperfusion in rats. Progress of Anatomical Sciences, 6: 358-360.
7.Hua F,Liu WR, Ding XH, Fang XB. (2001). Protective effect and mechanism of CGRP on transient ischemic cerebral tissue in rats.Modern Rehabitation, 5: 54-55.
8.Hua F,Ding XH, Liu WR, Fang XB. (2001). A novel model of whole cerebral ischemia / reperfusion induced by carotid artery negative pressure shunt in rats.Acta Laboratorium Animalis Scientia Sinica, 9: 33-39.
9.Hua F, Fang XB, Zhang L. (2002). Effect of CGRP and NGF on expression of BCL-2 in cortex and hippocampus response to whole cerebral ischemic/reperfusion in rats.Journal of brain and nerve system disease, 10: 269-274.
10.Hua F, Fang XB, Zhang L. (2002). The protective of CGRP on ischemic and reperfusion brain tissue in rats.Stroke and Nerve System Disease,9: 331-334.
11.Hua F, Fang XB, Lu Y. (2003). The dynamic change of CPP32mRNA in hippocampus after transient cerebral ischemia in rats.Clin Neurol., 16: 75-77.
12. Williams CA, Ricketts BA,Hua F, Dun NJ. (2002). Endomorphin-2 is not released from rat spinal dorsal horn in response to intraplantar formalin.Neurosci Lett.334: 5357.
13.Hua F, Ricketts BA, Reifsteck A, Ardell JL and Williams CA. (2003). Myocardial ischemia induces the release of substance P from cardiac afferent neurons in rat thoracic spinal cord.Am J Physiol Heart Circ Physiol.286:H1654-1664.
14.Hua F, Harrison T, Qin C, Reifsteck A, Ricketts B, Carnel C and Williams CA. (2004). c- Fos expression in rat brain stem and spinal cord in response to activation of cardiac ischemia-sensitive afferent neurons and electro stimulatory modulation.Am J Physiol Heart Circ Physiol.; 287:H2728-2738.
15.Hua F, Ardell JL. and Williams CA. (2004). Left vagal stimulation induces dynorphin release and suppresses substance P release from the rat thoracic spinal cord during cardiac ischemia.Am J Physiol Regul Integr Comp Physiol.287:R1468-1477.
16. Ha T, Li Y,Hua F, Ma J, Gao X, Kelly J, Zhao A, Haddad GE, Williams DL, Browder IW, Kao RL and Li C. (2005). Reduced cardiac hypertrophy in toll-like receptor4 deficient mice following pressure overload.Cardiovasc Res.68:224-234.
17.Hua F, Ha T, Ma J, Gao X, Kelley J, Williams DL, Browder IW, Kao RL and Li C. (2005). Blocking MyD88-dependent pathway protects the myocardium from ischemia/reperfusion injury in rat hearts.Biochem Biophys Res Commun.338:1118-1125.
18. Ha T,Hua F,Li Y, Ma J, Gao X, Kelly J, Zhao A, Haddad GE, Williams DL, Browder IW, Kao RL and Li C. (2006). Blocking of MyD88 attenuates cardiac hypertrophy and decreases cardiac myocyte apoptosis in pressure overload induced cardiac hypertrophy in vivo.Am J Physiol Heart Circ Physiol.290:H985-994.
19. Ha T,Hua F, Grant D, Xia Y, Ma J, Gao X, Kelley J, Williams DL, Kalbfleisch J, Browder IW, Kao RL, Li C. (2006). Glucan phosphate attenuates cardiac dysfunction and inhibits cardiac MIF expression and apoptosis in septic mice.Am J Physiol Heart Circ Physiol.291:H1910-918.
20. Hua F*, Ma J, Li Y, Ha T, Xia Y, Kelley J, Williams DL, Browder IW, Schweitzer JB, Li C. (2006). The development of a novel mouse model of transient global cerebral ischemia.Neurosci Lett.400:69-74. *corresponding author
21. Hua F,Ha T, Ma J, Li Y, Kelley J, Gao X, Browder IW, Kao RL, Williams DL and Li C. (2007). Protection against myocardial ischemia/reperfusion injury in TLR4 deficit mice is mediated through a phosphoinositide 3-kinase dependent mechanism.J Immunol.178:7317-7324.
22. Hua F, Ma J, Ha T, Xia Y, Kelley J, Williams DL, Kao RL, Browder IW, Schweitzer JB, Kalbfleisch J and Li C. (2007). Activation of Toll-like receptor 4 signaling contributes to hippocampal neuronal death following global cerebral ischemia/reperfusion.J Neuroimmunol.190: 101-111.
23. Ding X, Ardell J,Hua F,Daniel J, McAuley R and Williams C. (2008). Modification of cardiac ischemia-sensitive afferent neuron signaling by cervical spinal cord stimulation: effect on release of substance P from thoracic spinal cord.Am J Physiol Regul Integr Comp Physiol.294:R93-101.
24.Ha T,Hua F, Liu X, Ma J, Kelley J, Williams DL, Kao RL and Li C. (2008). Lipopolysaccharide preconditioning reduces myocardial ischemia/reperfusion injury through a PI3K/Akt dependent mechanism.Cardiovasc Res.78: 546-553.
25. Hua F, Ma J, Ha T, Kelley J, Williams DL, Kao RL, Kalbfleisch J, Browder IW and Li C. (2008). Preconditioning with a TLR2 specific ligand increases resistance to cerebral ischemia/reperfusion injury.J Neuroimmunol. 199: 75-82.
26. Ding X, Hua F, Sutherly K, Ardell JL, Williams CA. (2008). C2 Spinal Cord Stimulation Induces Dynorphin Release from Rat T4 Spinal Cord: Potential Modulation of Myocardial Ischemia-Sensitive Neurons. Am J Physiol Regul Integr Comp Physiol. 295, R1519-1528.
27. Hua F*, Ma J, Ha T, Kelley J, Kao RL,Schweitzer JB,Kalbfleisch J, Williams DL and Li C. (2009).Differential roles of TLR2 and TLR4 in acute focal cerebral ischemia/reperfusion injury in mice. Brain Res. 1262, 100-108.*corresponding author
28. Hua F*, Wang J, Sayeed I, Ishrat T, Atif F, Stein DG. (2009). The TRIF-dependent signaling pathway is not required for acute cerebral ischemia/reperfusion injury in mice. Biochem. Biophys. Res. Commun. 390, 678-683. *corresponding author
29. Lu C, Hua F, Liu L, Ha T, Kalbfleisch J, Schweitzer J, Kelley J, Kao R, Williams D, Li C. (2010).Scavenger receptor class-A has a central role in cerebral ischemia-reperfusion injury. J Cereb Blood Flow Metab. 30:1972-1981.
30. Ha T, Lu C, Liu L, Hua F, Hu Y, Kelley J, Singh K, Kao RL, Kalbfleisch J, Williams DL, Gao X, Li C. (2010). TLR2 ligands attenuate cardiac dysfunction in polymicrobial sepsis via a phosphoinositide 3-kinase-dependent mechanism. Am J Physiol Heart Circ Physiol. 298:H984-991.
31. Ishrat T, Sayeed I, Atif F, Hua F, Stein DG. (2010). Progesterone and allopregnanolone attenuate blood-brain barrier dysfunction following permanent focal ischemia by regulating the expression of matrix metalloproteinases. Exp Neurol. 226:183-190.
32. Hua F*, Wang J, Ishrat T, Wei W, Atif F, Sayeed I, Stein DG. (2011). Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone. J Neuroinflamm. 8:42. *corresponding author
33. Hua F*, Wang J, Ishrat T, Wei W, Atif F, Sayeed I, Stein DG. Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone. J Neuroinflammation. 2011;8:42. *corresponding author
34. Ishrat T, Sayeed I, Atif F, Hua F, Stein DG. Progesterone is neuroprotective against ischemic brain injury through its effects on the phosphoinositide 3-kinase/protein kinase B signaling pathway. Neuroscience. 2012;210:442-450.
35. Hua F, Reiss JI, Tang H, Wang J, Fowler X, Sayeed I, Stein DG. Progesterone and low-dose vitamin D hormone treatment enhances sparing of memory following traumatic brain injury. Horm Behav. 2012;61(4):642-51.
36. Cui G, Ye X, Zuo T, Zhao H, Zhao Q, Chen W, Hua F*. Chloroquine pretreatment inhibits toll-like receptor 3 signaling after stroke. Neurosci Lett. 2013;548:101-104. *corresponding author
37. Tang H, Hua F, Wang J, Sayeed I, Wang X, Chen Z, Yousuf S, Atif F, Stein DG. Progesterone and vitamin D: Improvement after traumatic brain injury in middle-aged rats. Horm Behav. 2013;64(3):527-538.
38. Atif F, Yousuf S, Sayeed I, Ishrat T, Hua F, Stein DG. Combination treatment with progesterone and vitamin D hormone is more effective than monotherapy in ischemic stroke: the role of BDNF/TrkB/Erk1/2 signaling in neuroprotection. Neuropharmacology. 2013;67:78-87.
39. Cui G*, Wang X, Ye X, Zu J, Zan K, Hua F*. Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence. Neural Regen Res. 2013; 8(34):3233-3240. *corresponding author
40. Hua F*, Tang H, Wang J, Prunty MC, Hua X, Sayeed I, Stein DG. TAK-242, an antagonist for Toll-like receptor 4, protects against acute cerebral ischemia/reperfusion injury in mice. J Cereb Blood Flow Metab. 2015 Jan 14. [Epub ahead of print]. *corresponding author
41. Yang X, Zhao H, Shi H, Wang X, Zhang S, Zhang Z, Zu J, Zhang W, Shen X, Cui G, Hua F*. Intranigral administration of substance P receptor antagonist attenuated levodopa-induced dyskinesia in a rat model of Parkinson's disease. Exp Neurol.2015 May 20;271:168-174. *corresponding author
申请专利：
1.TLR4拮抗剂保护小鼠脑缺血再灌注损伤；排名第一，美国，US14/173,942.
2.黄体酮通过调节炎症反应减轻新生小鼠脑缺血缺氧后癫痫发生；排名第三，美国,US61/717,821.
